Replication

Initiation

-Helicase unwinds double stranded DNA into single strands
-at the same time DNA gyrase is working at the end of each replication fork to ease off the tension produced
-Single Stranded binding protein comes and blocks the single stranded DNA from forming hydrogen bonds AGAIN
-RNA primase comes and produces RNA primers 

Elongation
-RNA primers initiate DNA Polymerase 3 to elongate in the 5-->3 direction
 -At the replication fork there will be a leading (moving towards the replication fork) and a lagging strand (moving away from replication fork)
-leading strand will be continuously made, while the lagging strand will make it in fragments called okazaki fragments
- During the process of elongating the lagging strand, DNA Polymerase 3 must wait until the replication fork opens little by little until it can continue to elongate. Therefore it needs additional RNA primers to initiate this process.
-When each okazaki fragment is made, DNA Polymerase 1 will come and remove the RNA primers and replace it with the corresponding bases
-DNA ligase then joins the okazaki fragments by adding phosphodiester bonds (attaching 5 prime to 3 prime...Phosphate group + OH)