Enzyme Lab

Procedure

1. Grind liver

2. Add  identical filter paper discs to the grinned liver

3. Pick out 35 identical filter paper discs (5 per run)

4. Dilute the acid and the base with different amounts of water to get different pH levels

5. Add 1 mL of NaOH to test tube #1, 2mL of NaOH to test tube #2, 3mL of NaOH to test tube #3

6. Add 1mL of HCl to test tube #4, 2mL to test tube #5, 3mL to test tube #6 

7. Add 4mL of water to test tube # 1 and 4, 3mL into test tube #2 and 5, and 2mL into test tube #3 and 6 

8. Thenn add 5 mL of hydrogen peroxide to each test tubes

9. Drop 5 filter paper discs in every Erlenmeyer flask for every run

10. Downward displacement

11. Watch for the rate of reactions ( bubbles) and look for amount of water displaced by oxygen

Results

TABLE:

Acidic Solution
Amount of HCl in(mL) added	1mL of HCl	2mL of HCl	3mL of HCl	5mL of HCl
pH level of the Solution	pH 5-7	pH 1-2	pH 1-2	pH 1
Colour of Litmus Paper	Yellow litmus	Orange/ red litmus	Red litmus	Red litmus
Amount of Water Displaced by Oxygen in (mL) and Qualitative Observation	Bubbles formed around filtrate paper, however no waters displaced	Bubbles formed around filtrate paper very quick. Approximately 5mL of water was displaced	Bubbles formed vigorously around filtrate paper. Approximately 6mL of water was displaced	Oxygen displaced as soon as filtrate paper touched solution and filtrate paper bubbled as well. Approximately 10mL of water was displaced.

Basic Solution
Amount of NaOH in (mL) added	1mL of NaOH	2mL of NaOH	3mL of NaOH
pH level of the Solution	pH 9	pH 9-11	pH 11
Colour of Litmus Paper	Blue/green litmus	Blue litmus	Dark blue litmus
Amount of Water Displaced by Oxygen in (mL) and Qualitative Observation	115mL of water displaced	11 mL of water displaced	2mL of water displaced